IN VITRO MICROPROPAGATION OF THE ORCHID (Dendrobium crystallinum var. alba)

IN VITRO MICROPROPAGATION OF THE ORCHID (Dendrobium crystallinum var. alba)

Tran Thi Ngoc Lan

ABSTRACT

Studies of micropropagation of Dendrobium crystallinum var. alba. Were conducted in order to conserve and develop this precious orchid species. The results showed that protocorms were formed from seeds culture on the ½ MS medium supplemented with 10% potatoes extract within 8 weeks. Protocorms which were cultured on the ½ MS medium containing 30 g/l sucrose, 0.5 g/l AC, 7 g/l agar, 0.1 mg/l NAA and 1 mg/l BA were optimal for PLB formation (8.67 PLBs/sample) after 8 weeks culture. Protocorms converted into normal plants with well-developed shoots and roots on the ½ MS medium supplemented with 20 g/l sucrose, 10% potatoes extract, 0.5 g/l AC, and 7 g/l agar after about 90 days. PLBs converted into normal plants on the same medium supplemented with 0.5 mg/l NAA and 0.5 mg/l BA, too. No abnormal morphological changes were found in these Dendrobium seedlings.

AGU International Journal of Sciences – 2019, Vol 7 (3), 1 – 8