GENETIC DIVERSITY OF NATURALLY DISTRIBUTED Rhododendron moulmainense Hook. f. POPULATIONS IN LAM VIEN PLATEAU, VIETNAM REVEALED BY ISSR AND SCoT MARKERS

GENETIC DIVERSITY OF NATURALLY DISTRIBUTED Rhododendron moulmainense Hook. f. POPULATIONS IN LAM VIEN PLATEAU, VIETNAM REVEALED BY ISSR AND SCoT MARKERS

Luu The Trung¹, La Anh Duong², Tran Van Tien³, Le Ngoc Trieu³ và Phi Hông Hai^2*

¹Forest Science Institute of central Highlands and South of central Viet Nam

²Vietnamese Academy of Forest Science

³Faculty of Biology, Dalat University

Abstract

Genetic diversity and variation of three naturally distributed Rhododendron moulmainense populations in the Lam Vien Plateau of Vietnam were analyzed using ISSR and ScoT markers separately and data from the two types of markers were then combined to show the overall results. Based on combined data, the genetic diversity parameters of the Tuyen Lam population were percentage of polymorphic bands (PPB) = 28.79%; genetic heterozygosity (He) = 0.1041; Shannon index (I) = 0.1552. The corresponding parameters were PPB = 37.88%; He = 0.1434; I = 0.2131 in the Hon Nga population, and PPB = 46.21%; He = 0.1857; I = 0.2709 in the Bidoup population. At the species level in Lam Dong province, the corresponding parameters were PPB = 62.12%; He = 0.1747; I = 0.2756. The average of pairwise genetic similarity coefficients among investigated individuals of Tuyen Lam population was 0.973, of Hon Nga population was 0.911, and of Bidoup population was 0.895. The genetic differentiation and the number of migrants per generation among investigated populations were GSTS = 0.1735 and Nm = 2.3824 individuals, respectively. Genetic distance between Tuyen Lam and Hon Nga populations was 0.0399, between Tuyen Lam and Bidoup populations were 0.0621, and Bidoup and Hon Nga populations were 0.0670. Data generated by both marker types and the combined revealed similar trends of genetic diversity levels among the studied populations. The SCoT data showed higher values of genetic diversity and population gene differentiation than did the ISSR data, but the latter showed higher gene differentiation among sampled individuals in the study populations. Comparing the values of Polymorphism Information Content (PIC) for discriminatory ability; Effective Multiplex Ratio (EMR) for several revealed loci and/or efficiency of the primer-marker system; Marker Index (MI) for the efficiency of used method; and Resolving Power (Rp) between used techniques indicated that discriminatory ability, efficiency, and ability of the ISSR primer combinations were higher but using ScoT marker system was more efficient than ISSR marker system for evaluating genetic diversity.

Key words: Rhododendron moulmainense, ISSR, SCoT genetic diversity, Vietnam

Sources: Malays. Appl. Biol. (2020) 49(5): 41–52

Fig. 3. Distribution of three investigated R. moulmainense populations